85-1573-81　［取扱停止］Rsk1, active, Recombinant, Rat (Ribosomal S6 Kinase 1, RSK-1, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP kinase 1a, Mapkapk1a, MAPKAPK-1a, 90kD Rbosomal Protein S6 Kinase 1, p90RSK1, p90-RSK 1, p90S6K, Ribosomal Protein S6 Kinase alpha-1, Rps6ka1, S6K-alpha-1) 10ug　R9399-08A

特徴

• This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Recombinant full-length rat RSK1 (residues 1–end), containing additional N-terminal 6His and HA (YPYDVPDYA) tags, expressed in baculovirus in Sf21 insect cells
• Specific Activity:~4000-5000U/mg
• Unit Definition:One unit of RSK1 activity is defined as 1nmol phosphate incorporated into 30uM Rsktide/minute at 30°C with a final ATP concentration of 100uM.
• Quality Control Testing:Kinase Assay: 0.3–3.8ng phosphorylates 30uM RSKtide. Assay background was subtracted from the actual counts to yield the results shown below
• MS Tryptic Fingerprint: Confirmed product identity as rat RSK1 with the translated sequence. SDS-PAGE and Coomassie Stain: Purity was assessed by SDS-PAGE and Coomassie blue staining using 3ug of RSK1 active.
• Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Handling Recommendations: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.