ntegrase is an enzyme, a product of the HIV1 Gag/Pol gene; the other two enzymes being reverse transcriptase and protease. The human immunodeficiency virus (HIV-1) uses an enzyme, a so-called integrase, to carry out the integration of its viral DNA into the host chromosome thereby tricking the host cell machinery into making viral proteins. The HIV-1 integrase is a protein of 32kD and is composed of three domains.
No cellular homologue of HIV integrase has been described, so potential inhibitors to this enzyme could be relatively nontoxic.
Source:Recombinant corresponding to HIV-1 immunodominant regions from integrase p31 (aa9-289) fused to 6-His-tag expressed in E. coli.
Applications:Suitable for use in ELISA, Lateral Flow and Western Blot. Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E. coli
Grade：Highly Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a liquid in 25mM Tris-HCl, 1.5M urea, pH 8.0, 0.02% Triton-X, 50% glycerol.
