85-1573-32　RNMT, Recombinant, Human aa1-476, His-Tag (mRNA Cap Guanine-N7 Methyltransferase, mRNA (guanine-N(7)-)-Methyltransferase, RG7MT1, mRNA Cap Methyltransferase, hcm1p, hCMT1, hMet, KIAA0398) 50ug　R2041-70C

特徴

• RNMT (mRNA cap guanine-N7 methyltransferase), also known as MET, RG7MT1 or hCMT1c, is a widely expressed nuclear protein that belongs to the mRNA cap methyltransferase family. Cap-dependent mRNA translation requires the methylation of the mRNA guanosine cap by RNMT. RNMT catalyzes the transfer of a methyl group from AdoMet (S-adenosylmethionine) to the GpppN end of the growing mRNA at the N-7 position, thereby producing AdoHyc (S-adenosylhomocysteine) and m7GpppN terminated RNA.
• Source:Recombinant corresponding to aa1-476 of human RNMT, fused with His-tag at N-terminus, expressed in E.coli.
• AA sequence:MGSSHHHHHH SSGLVPRGSH MANSAKAEEY EKMSLEQAKA SVNSETESSF NINENTTASG TGLSEKTSVC RQVDIARKRK EFEDDLVKES SSCGKDTPSK KRKLDPEIVP EEKDCGDAEG NSKKRKRETE DVPKDKSSTG DGTQNKRKIA LEDVPEKQKN LEEGHSSTVA AHYNELQEVG LEKRSQSRIF YLRNFNNWMK SVLIGEFLEK VRQKKKRDIT VLDLGCGKGG DLLKWKKGRI NKLVCTDIAD VSVKQCQQRY EDMKNRRDSE YIFSAEFITA DSSKELLIDK FRDPQMCFDI CSCQFVCHYS FESYEQADMM LRNACERLSP GGYFIGTTPN SFELIRRLEA SETESFGNEI YTVKFQKKGD YPLFGCKYDF NLEGVVDVPE FLVYFPLLNE MAKKYNMKLV YKKTFLEFYE EKIKNNENKM LLKRMQALEP YPANESSKLV SEKVDDYEHA AKYMKNSQVR LPLGTLSKSE WEATSIYLVF AFEKQQ
• Molecular Weight:~57kD (496aa)
• Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.