NAT1 belongs to the arylamine N-acetyltransferase family. It catalyzes N- or O-acetylation of heterocyclic and arylamine substrates in the detoxification of a wide array of drugs. Certain alleles causing high levels of N-acetyltransferase activity have been associated with colon and urinary bladder cancers, as NAT1 also bioactivate several known carcinogens. This enzyme helps metabolize drugs and other xenobiotics, and participates in the detoxification of a plethora of hydrazine and arylamine drugs. Purified by using conventional chromatography techniques.
Source:Recombinant corresponding to aa1-290 of human NAT1, fused to His-tag at N-terminal, expressed in E.coli.
Molecular Weight:~36.1kD (310aa), confirmed by MALDI-TOF
Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E.coli
Grade：Purified
Purity：~85% (SDS-PAGE)
Form：Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 2mM DTT, 0.1M sodium chloride, 10% glycerol.
