Nth, also known as Endonuclease III, has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme incises damaged DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5' from the damaged site. This enzyme is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
Description:Recombinant E. coli NTH (Endonuclease III) protein fused to His-tag at the N-terminus; expressed in E. coli
Molecular Weight:25.7kD (231aa) confirmed by MALDI-TOFEnzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C or lower. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：250ug
Source Antigen：Recombinant, E. coli
Grade：Purified
Purity：≥ 85% (SDS-PAGE)
Form：Supplied as a liquid in 20mM Tris-HCl buffer, pH8.0, 0.1M sodium chloride, 1mM DTT, 0.1mM PMSF, 40% glycerol.
