TSEN15 (tRNA-splicing endonuclease subunit Sen15) belongs to the SEN15 family. tRNA splicing is a fundamental process required for cell growth and division. SEN15 is a subunit of the tRNA splicing endonuclease. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron
Purified by using conventional chromatography techniques.
Source:Recombinant human corresponding to aa1-171 of TSEN15, fused to His-tag at N-terminus, expressed in E.coli.
AA Sequence:MGSSHHHHHH SSGLVPRGSH MEERGDSEPT PGCSGLGPDG VRGFGDGGGA PSWAPEDAWM GTHPKYLEMM ELDIGDATHV YVAFLVYLDL MESKSWHEVN CVGLPELQLI CLVGTEIEGE GLQTVVPTPI TASLSHNRIR EILKASRKLQ GDPDLPMSFT LAIVESDSTI VYYKLTDGFMLPDPQNISLR R
Molecular Weight:~20.9kDa (191aa) confirmed by MALDI-TOF
Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：50ug
Source Antigen：Recombinant, E.coli
Grade：Highly Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 2mM DTT, 200mM sodium chloride, 20% glycerol.
