MPG (DNA-3-methyladenine glycosylase) belongs to the DNA glycosylase MPG family. This protein initiates base excision repair in DNA by removing a wide variety of alkylated, deaminated, and lipid peroxidation-induced purine adducts. Purified by using conventional chromatography techniques.
Source:Recombinant corresponding to aa1-298 of human MPG, fused to His-tag at C-terminal, expressed in E.coli.
Molecular Weight:~33.9kD (306aa), confirmed by MALDI-TOF
Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:50ug
Source Antigen:Recombinant, E.coli
Grade:Highly Purified
Purity:~ 90% (SDS-PAGE)
Form:Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 1mM DTT, 200mM sodium chloride, 1mM EDTA, 30% glycerol.