ADP-ribosylation factor 5, also known as ARF5, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of actin to the golgi via rho family-dependent G-protein localization and WASP/Arp2/3 complexes. ARF5 is involved in vesicular transport and functioning via phospholipase D activation. Purified by using conventional chromatography techniques.
Source:Recombinant corresponding to aa1-180 of human ARF5, fused to His-tag at N-terminal, expressed in E.coli.
Molecular Weight:~22.6kD (200aa), confirmed by MALDI-TOF
Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Recombinant, E.coli
Grade：Highly Purified
Purity：~90% (SDS-PAGE)
Form：Supplied as a liquid. In 20mM Tris-HCl buffer, pH 8, 1mM DTT, 0.1M sodium chloride, 20% glycerol.
