Heparan sulfate is a sulfated glycosaminoglycan with the repeating disaccharide units of 4HexA1, 4GlcNAcB1. It is usually attached to the protein cores of proteoglycans found on cell membrane and extracellular matrix, where it binds to a variety of protein ligands and regulates a wide range of biological activities, including developmental processes, angiogenesis, blood coagulation and tumor metastasis (1, 2). Heparan sulfate has a domain structure containing sulfated regions interspaced with less or nonsulfated regions (3, 4). Heparin shares the backbone structure with heparan sulfate but contains no nonsulfated regions. Heparinases are a family of lyases that release unsaturated oligosaccharides from heparin and heparan sulfate upon digestion (5). Heparinase I recognizes highly sulfated regions and is more specific for heparin. Heparinase II digests both heparin and heparan sulfate. Heparinase III prefers less sulfated regions and is active only on heparan sulfate (6, 7).
Source:E. coli-expressed recombinant protein corresponding to Ala26-Arg772 of P. heparinus Heparinase II, with an N-terminal Met and 6-His tag
Specific Activity:Measured by its ability to liberate oligosaccharides from heparin. The specific activity is >750 pmoles/min/ug, as measured under the described conditions (see Activity Assay Protocol)
Endotoxin Level:<1EU/ug (LAL method)
Storage and Stability:Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：10ug
Source Antigen：Recombinant, E.coli
Grade：Highly Purified
Purity：≥ 95% (SDS-PAGE, reducing conditions; 5ug protein loaded per lane; visualized by colloidal Coomassie blue staining)
Form：Supplied as a sterile-filtered liquid in Tris-buffered saline
