NADPHCytochrome P450 Reductase (P450R) is an essential component of the cytochrome P450 monooxygenase system of eukaryotic cells (1). P450R is anchored in the endoplasmic reticulum membrane with its catalytic domain residing in the cytosol. P450R is a flavoprotein, containing one molecule each of FMN and FAD, which are essential for the transfer of electrons from NADPH to the cytochromes P450 (2). This reduction is necessary for cytochromes P450 to perform each cycle of oxidation. P450R is also capable of transferring electrons to cytochrome b5, heme oxygenase, the fatty acid elongation system, and other proteins. Mutations of P450R can result in disordered steroidogenesis and AntleyBixler syndrome.
Recombinant protein corresponding to Arg45-Ser677 from human P450R, N-terminal, human CD33 signal sequence and 6-His tag, expressed in Spodoptera frugiperda, Sf 21 (baculovirus).
Biological Activity:Measured by the reduction of cytochrome c using NADPH as the cofactor. The specific activity is >6,500 pmoles/min/ug, as measured under the described conditions.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
