85-1571-27　［取扱停止］Normetanephrine, Human, BioAssay™ ELISA Kit 96Tests　N4000-38H

特徴

• Sample Type:Urine
• Intended Use:Enzyme immunoassay for the manual and automated quantitative determination of normetanephrine in human urine.
• Test Principle:The assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and a non-biotinylated antigen for a fixed number of antibody binding sites. The amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of anti-biotin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is achieved by comparing the enzymatic activity of unknowns with a response curve prepared by using known standards.
• Kit Components:Assay Buffer, Concentrate (10X), 1x50mlAcylation Reagent, 1x2.25ml Indicator Buffer, 1x10mlHydrolyzation Tubes, 2x50eachHCl Solution, 0.1M HCl, 1x20ml Microtiter plate, Coated with anti-rabbit IgG (goat, polyclonal), 1x96 wellsStandard A-G (7x0.35ml)Standard, 0ug/L, 1x350ulStandard, 77ug/L, 1x350ulStandard, 192ug/L, 1x350ulStandard, 480ug/L, 1x350ulStandard, 1200ug/L, 1x350ulStandard, 3000ug/L, 1x350ulStandard, 7500ug/L, 1x350ulControl 1, 1x0.5mlControl 2, 1x0.5mlNormetanephrine Biotin, 3x1vialNormetanephrine Antiserum, 1x7mlEnzyme Conjugate, Concentrate (100X), 1x250 ulSubstrate Tablets, Contains: p-nitrophenyl phosphate (PNPP), 9x1eachSubstrate Buffer, 1x27ml Stop Solution, 1x15ml Wash Buffer, Concentrate (10X), 1x50ml
• Storage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.