Intended Use:Enzyme immunoassay for the quantitative determination of neopterin in human serum, plasma and urine.
Test Principle:Solid phase enzyme-linked immunosorbent assay (ELISA) based on the basic principle of a competitive ELISA. An unknown amount of antigen in the sample and a fixed amount of enzyme labelled antigen compete for the antibody-binding sites (rabbit-anti-neopterin). Both antigen-antibody complexes bind to the wells of the microtiter strips coated with a goat-anti-rabbit antibody. Unbound antigen is removed by washing. The intensity of the color developed after the substrate incubation is inversely proportional to the amount of antigen in the sample. Results of samples can be determined directly using the standard curve.
Materials Required But Not Supplied:1. Micropipettes (Multipette Eppendorf or similar devices, < 3% CV). Volumes: 10; 50; 100; 1000 IL2. Vortex mixer3. Orbital shaker (500rpm)4. 8-Channel Micropipettor with reagent reservoirs5. Wash bottle, automated or semi-automated microtiter plate washing system6. Microtiter plate reader capable of reading absorbance at 450nm (reference wavelength 600-650nm)7. Double-distilled or deionized water8. Paper towels, pipette tips and timer
Storage and Stability:Store all other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials prior to removing the cap.
