Intended Use:The HSV-2 IgG ELISA is intended for use in evaluating a patient’s serologic status to herpes simplex virus (HSV) type 2 infection, or for evaluating paired sera for the presence of a significant increase in herpes specific IgG.
Introduction:Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There are two immunologically distinct types of HSV: Type 1 and Type 2. HSV-1 is generally associated with oral infection and lesions above the waist, and HSV-2 is associated with genital infections and lesions below the waist. Clinical cases primarily are (1) eczema herpeticum with eczematous skin changes with numerous lesions, (2) gingivostomatitis, and (3) herpes sepsis, almost only found in premature newborn infants. The HSV-2 IgG ELISA is an accurate serologic method to detect HSV-2 IgG specific-antibody in serum samples.
Test Principle:Purified HSV-2 antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and the HSV-2 IgG-specific antibody, if present, binds to the antigen. All unbound materials are washed away. HRP-conjugate is added, which binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and a solution of TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of HSV-2 IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.
Storage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
