Intended Use:The HSV-1 IgG ELISA is intended for use in evaluating serologic status to herpes simplex virus (HSV) type 1 infection, or for evaluating paired sera for the presence of a significant increase in HSV-1 specific IgG.
Introduction:Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There are two immunologically distinct types of HSV: Type 1 and Type 2. HSV-1 is generally associated with oral infection and lesions above the waist, and HSV-2 is associated with genital infections and lesions below the waist. Clinical cases primarily are (1) eczema herpeticum with eczematous skin changes with numerous lesions, (2) gingivo-stomatitis, and (3) herpes sepsis, almost only found in premature newborn infants. The HSV-1 IgG ELISA is an accurate serologic method to detect HSV-1 IgG specific-antibody in serum samples.
Test Principle:Purified HSV-1 antigen is coated on the surface of microwells. Diluted serum is added to the wells, and the HSV-1 IgG specific-antibody, if present, binds to the antigen. All unbound materials are washed away. HRP-conjugate is added, which binds to the antibody-antigen complex. Excess HRP-conjugate is washed off and a solution of TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of HSV-1 IgG-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.
Kit Components:Antigen-Coated Wells (1 plate, 96 wells), Microtiter Wells coated with purified HSV-1 antigenEnzyme Conjugate Reagent (12ml), Contains goat anti-human IgG conjugated to horseradish peroxidase with preservativesSample Diluent (22ml), Contains phosphate buffer with preservativesNegative Control (150ul/vial), Contains HSV-1 IgG negative serum with preservativesCut-off Calibrator (150ul/vial), Contains HSV-1 IgG weak positive serum with preservatives; HSV-1 IgG Index = 1Positive Control (150ul/vial), Contains HSV-1IgG positive serum with preservativesWash Buffer Concentrate (20X) (50ml), Contains phosphate buffer and Tween 20TMB Reagent (11ml), Contains 3, 3’, 5, 5’ tetramethylbenzidine (TMB) stabilized in buffer solutionStop Solution -1N HCl (11ml), Diluted hydrochloric acid
Storage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
