The Human Complement C3 ELISA Kit is an in vitro immunoassay for the quantification of C3 in serum of other biological fluids. The kit performance has been optimized for the protocol and materials listed below using standard dilutions of human Complement C3 in the 0.5-500ng/ml range. The operator must determine appropriate dilutions of reagents for alternative assay conditions. ELISA assay reactivity is sensitive to variations in operator, pipetting and washing techniques, incubation time, temperature, composition of reagents, and other experimental variables. Assay optimization may be required to generate the standard curve and fit the samples in the specified detection range.
Kit Components (suitable for 10x96 tests):C7850-11H1: Coating Antibody (1.35mg/ml), 1x170ul, Affinity purified C3 Pab Ch x Hu, IgY, working dilution: 1:675C7850-11H2: Calibrator (0.56mg/ml), 1x25ul, Purified Human Complement C3 Antigen, Working dilution: 1:280C7850-11H3: Detection Antibody (HRP) (0.2mg/ml), 1x20ul, Affinity purified C3 (HRP) Pab Ch x Hu, IgY, Working dilution: 1:10,000
Detection Range: ~0.5-500ng/ml
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
