Omega-amidase NIT2, also known NIT2, belongs to the nitrilase superfamily. This protein has an omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Also Nit2, which is widely distributed in nature, has been suggested to be a tumor suppressor protein.
Source:Recombinant corresponding to human NIT2, fused to His-tag at N-terminus, expressed in E. coli.
Enzyme Activity:Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size:50ug
Source Antigen:Recombinant, E. coli
Grade:Highly Purified
Purity:~95% (SDS-PAGE)
Form:Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 10% glycerol, 0.1M sodium chloride.