85-1570-52　Hydroxyacyl Glutathione Hydrolase, aa1-260, Recombinant, Human (Hydroxyacylglutathione Hydrolase, HAGH, HAGH1, GLO2, Glyoxalase II, Glx II, GLXII, GLX2) 50ug　H9030-05

特徴

• HAGH is a member of the glyoxalase family and a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. This protein is a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73 and serves as a pro-survival factor of the p53 family. It exists only as a monomer and binds two zinc ions per subunit.
• Source:Recombinant corresponding to human HAGH, fused to His-tag at N-terminus, expressed in E. coli.
• Molecular Weight: ~31.4kD
• Sequences of Amino Acids:MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDPVQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKL VKLESGLKVY GGDDRIGALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAGCGKFYE GTADEMCKAL LEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGNAAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDPVTTMR AVRREKDQFK MPRD
• Enzyme Activity: Specific activity is > 500 units/mg, and is defined as the amount of enzyme that reduces 1.0 umole of 5,5 -Dithiobis(2-nitrobenzoic acid) (DTNB) with reduced glutathione per minute at pH 7.5 at 37°C.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.