85-1570-33　Western Blot ECL Kit with Enhancer, Anti-Mouse, BioAssay™ 30 Blots　W1019-82A

特徴

• Ultra-sensitive ECL Western Blot System that detects attogram (10e18g) or yoctomole (10e24M) protein levels. The Enhancer utilizes a proprietary technology that increases antigen and antibody affinity and accessibility which can work with different antigens and antibodies, such as structural proteins (Actin), enzymes (GAPDH) or membrane proteins (EGFR), increasing Western blot signals dramatically.
• Kit Components:W1019-82A1: Enhancer, 1x50ml or 1x100mlW1019-82A12 Antibody Binding Buffer, 20x, 1x60ml or 1x120mlW1019-82A3: Blocking Agent, 1x20g or 1x40gW1019-82A4: HRP Conjugated Secondary Antibody (anti-Mouse), 1x50ul or 1x100ulW1019-82A5: Luminescence Substrate solution A, 1x25ml or1x100mlW1019-82A6: Luminescence Substrate solution B, 1x25ml or 1x50mlStorage and Stability:May be stored at 4°C. Stable for 6 months after receipt.
• Two convenient and affordable sizes:15 Mini Blots (1200cm2) and 30 Mini Blots (2500cm2).
• Quality Control:Duplicated Western blots containing 10fg and 10pg of antigen are detected with anti-GAPDH antibody. Antigen at 10fg levels was detected only on the blot treated with Enhancer, with similar intensity as antigen at 10pg level on the untreated blot.
• One ng of purified Rabbit muscle GAPDH was serially diluted to 1 attogram and electrophoresis was performed. The gel was transferred to a PVDF membrane, and then cut in the middle. The right section was treated with Enhancer, and the left was untreated. Immunoblotting was done using a primary antibody and HRP-conjugated rabbit anti-mouse antibody, and chemiluminescent substrates were applied. A film exposed for two minutes is shown above. 10-e11g of antigen without treatment and 10e14g of GAPDH with treatment gave similar signal strengths, indicating a 1000-fold enhancement*
• * Fold enhancement varied from a few to a 1000-fold depending on the characteristics of antibodies and antigen used.
• Note:Components in this kit are free of precipitate. When testing by visible spectrophotometry, the color of the solution is comparable to a control Lot. Results from each lot are comparable to those obtained with control lot of the solution.