BioGenomics™ Tissue cDNA:cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.
BioGenomics™ Universal cDNAMixed Male and Female donors. Prepared by reverse transcribed RNA by using random hexamer or oligo dT primer. Total RNA is isolated by modified guanidine thiocyanate techniques. The Universal cDNA serves as a standard for comparison of gene expression by real time PCR and regular PCR, and also as a gene pool for cloning genes.
Applications:Suitable for use in SNP analysis, Southern Blot, PCR, Genomic DNA library construction, Profiling study in gene expression
Species Selection:Choose from Human, Mouse, Rat, Chicken, Canine, Monkey.
Human Universal cDNA is prepared from major organs of both male and female adult donors
Mouse Universal cDNA is prepared from several male and female Balb/C mice whole bodies without fur.
Rat Universal cDNA is prepared from several male and female Sprague Dawley or Wistar rats whole bodies without fur.
Chicken (Leghorn) Universal cDNA is prepared from major organs of both male and female adult donors.
Canine (Beagle) Universal cDNA is prepared from major organs of both male and female adult donors
Monkey (Cynomolgus and Rhesus) Universal cDNA is prepared from major organs of both male and female adult donors
Supplied With:T5595-0010: Tissue, cDNA, Actin Control Primer
Concentration:2.5ng/ul; 1ul cDNA is sufficient for one PCR reaction.
Form:Supplied as a liquid in 1X RT buffer: 50mM Tris-Cl, pH 8.3, 75mM KCl, 3mM MgCl2, 10mM DTT.
Control PCR Component:Taq polymerase0.2ul10X PCR buffer2.5ul10mM dNTP0.5ulControl primers (5uM)1.0ulPCR Ready First Strand cDNA1.0ulSterile ddH2O19.8ulTotal Volume25.0ul
Control PCR Conditions:1. 94°C x 2 minutes, 1 cycle
2. 94°C x 30 seconds, 55°C x 30 seconds, 72°C x 30 seconds, 35 cycles
3. 72°C x 5 minutes, 1 cycle. Hold at 4°C.
Quality Control:RNA Integrity for cDNA synthesis examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA using denaturing agarose gel. Quality and purity of total RNA is determined spectrophotometrically
. RNA used for cDNA synthesis is treated by DNase I and is tested as DNA-free RNA by PCR.
The synthesized human, animal, and cell line cDNA was 5’ selected to ensure its full length.
The cDNA was used as template for PCR amplification of beta-actin gene and an 838 bp beta-actin band was visualized on 1% agarose gel
Synthesized plant cDNA was used as template for PCR amplification of chloroplast gene.
458bp chloroplast band visualized on 1% agarose gel
β-actin control primer included, 10 PCR reactionsChloroplast control primer included., 10 PCR reactions
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
