Description:All the protein lysates were prepared from liquid nitrogen fresh frozen tissues. Total protein and universal protein lysates were prepared by a Total Protein Extraction Kit (Cat# P9087).
Human tumor protein matched products include: Primary Pair (PP), or Primary and Metastatic Pair (PM)
PP consists of total protein isolated from primary tumor and its adjacent normal tissue; PM consists of total protein from primary tumor and corresponding metastastic tumor. Total proteins in each pair are prepared from the same donor. This product line is designed for identifying tumor-specific genes and tumor metastatic genes
Quality Control:1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining, the pattern is consistent with each lot
2. The isolated protein is analyzed by Western Blot using an antibody to • GAPDH for total protein, cytoplasmic and universal total protein.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for at least 6 months. After the protein is thawed, store it at 4°C for continuous use. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2x200ug
Source Antigen：Human
Grade：Molecular Biology Grade
Form：Supplied in a buffer including HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
