The Cartilage RNA Isolation Kit contains 4 solutions specifically for RNA isolation from different cartilage tissues. The isolated cartilage total RNA can be used for cDNA library construction, subtracted probe generation, RT-PCR, Northern blot analysis, primer extension, RNA protection assay, and In vitro translation. This kit can also be used for isolation of total RNA from other kinds of tissues.
Materials Required But Not Supplied:Isopropanol100% Ethanol70% EthanolChloroform
Storage and Stability:Store T5595-0003A and T5595-0003B at 4°C. Store the rest of the kit components at room temperature. If precipitate forms in T5595-0003E, place the bottle in a 65°C water bath to dissolve it before use. Kit is stable for 6 months.
Quality Control:Mouse Cartilage total RNA was isolated from each lot:1.The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA via electrophoreses on a denaturing agarose gel. 2. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH 7.5). .3. The RNA is treated by DNase I, and is tested as DNA free RNA by PCR. 4. cDNA synthesis is successfully performed by using this RNA as template.
