Unit Definition:One unit is the amount of enzyme that incorporates I nmole of [3H] GMP into acid-insoluble fraction in 1 hour at 37°C and pH 8.
Purity:Nuclease activity was not detected in any of the following cases, as judged from the intact gel electrophoresis:1. After incubation of 1ug of λ DNA-Hind III fragments with 500 units of enzyme for 24 hours at 37°C2. After incubation of 1ug of pBR322 DNA with 500 units of enzyme for 24 hours at 37°C3. After incubation of 1ug of 16S and 23S rRNA with 500 units of enzyme for 24 hours at 37°C This enzyme was more than 95% homogeneous as judged from SDS-polyacrylamide gel electrophoresis.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
