85-1563-89　Ribonuclease H (RNase H) discontinued 100U　R2021-01

特徴

• Storage Buffer:100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100 and 50% glycerol.
• Unit Definition:One unit of Ribonculease H will produce 1 nmol of acid-soluble ribonuleotides from radiolabeled poly(rA): poly (dT) in 20 minutes at 37°C.
• Assay Conditions:20mM Tris (pH 7.5), 5mM MgCl2, 2mM DTT, 0.006% BSA
• Quality Control: Endonuclease/Nickase Activity: None detected after a 14-16 hour incubation of 5 units of enzyme with 300ng of supercoiled plasmid DNA, analyzed on an agarose gel.
• Exonuclease Activity:None detected after a 14-16 hour incubation of 5 units of enzyme with 40ng of 32P labeled Sau3A fragments of pUC19, analyzed by PAGE..
• RNase Activity:None detected after a 14-16 hour incubation of 5 units of enzyme with 25ng of 32P labeled RNA, analyzed by PAGE.
• Protease Activity:None detected after a 14-16 hour incubation of 60 units of enzyme with 1ug of protease substrate, analyzed by fluorescence.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.