Description:Components in this kit are prepared with pure chemicals according to the proprietary technology. To prevent protein degradation, a ready-to-use protease inhibitor cocktail is provided. Membrane Protein Extraction Kit is designed to quickly isolate membrane proteins from mammalian tissues and cells. The kit is consisted of reagents enough to enrich membrane proteins from 5 grams tissues or about 125 million cells. The protein product has been investigated by SDS-PAGE and immunoblotting of selected marker proteins.
Quality Control:The kit of this lot has been tested to go through the membrane protein extraction procedure from rat colon. The proteins from the extraction are used for electrophoresis, transferring to PVDF membrane and immunoblotting with Mouse anti-Na/K ATPase as primary antibodies, and HRP-conjugated anti-Mouse IgG as secondary antibodies.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
