Introduction:Cell death is assayed by the quantification of plasma membrane damage. One of the standard methods is the measurement of cytoplasmic enzyme activity released from damaged cells. The amount of enzyme activity correlates to the proportion of the damaged cells. LDH Cytotoxicity Assay kit is a colorimetric assay that quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released into the cell culture supernatant upon damage of the cytoplasmic membrane. Released LDH in culture supernatants is measured with a 20-minute coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be measured by 490 nm absorbance using a standard ELISA plate reader. The LDH-cytotoxicity assay is fast, sensitive, convenient, and precise, and is applicable to a variety of cytotoxicity studies.
Features:
Safe – No radioactive isotopes are required
Easy and Simple - Add solutions to the cells, incubate and read the absorbance. The entire assay is performed in one microtiter plate with no washing and cell harvesting.
Fast – Short reaction time, no centrifuge needed
Accurate – Absorbance strongly correlates the number of lysed cells.
Sensitive – More sensitive than alkaline and acid phosphatase-based assays.
Reliable – Super-quality and highly reproducible data
Applications:
Measurement of cytotoxic effects of cytotoxic and cytostatic compounds such as anti-cancer drugs and many other toxic drugs.
Can be used in fast, high-throughput drug screening.
Description:Components in this kit are prepared with pure chemicals according to our proprietary technology. LDH Cytotoxicity Assay Kit enables to measure the cytotoxicity with colorimetric assay and based on based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells. The procedures are simple, fast, sensitive and accurate. One kit is consisted of reagents enough for performing 400 assays.
Quality Control:1 kit of this lot has been tested to go through the complete cytotoxicity assay. The 490nm absorbance of cell supernatant using this lot of kit is comparable to those obtained with control (previous) lot.
Kit Components:LDH Cytotoxicity Assay Kit: Reagents are sufficient for 400 assays
1. Catalyst Reagent (containing Diaphorase and NAD+), 1x1vial2. Catalyst Reconstitution Buffer, 1x6ml3. Dye Solution (containing tetrozolium INT and lactate), 1x12ml4. Lysis Solution (10X), 1x5ml5. Stop Solution, 1x15ml
