ELISA Kit for Antibody to Human Immunodeficiency Virus 1&2 is an in vitro enzyme immunoassay for the detection of anti-HIV antibodies in human serum or plasma.
Principle:This kit uses two-antigen sandwich ELISA method to detect anti- HIV (1+2) antibodies in serum or plasma. The HIV antigens (gp 36 and gp 41) with high purity are coated on the multi-wells strips. When serum sample and HIVAg labeled with HRP (conjugated) are added to the coated wells, and if Anti-HIV is present in the sample, a complex of HIVAg-Anti-HIV-HIVAg labeled with HRP will form. The enzyme reaction with substrate TMB produces a color change, and the intensity of the absorbance at 450 nm indicates the presence or absence of Anti-HIV antibodies in the sample. The test is specific, sensitive, reproducible and easy to operate. It is for blood screen of HIV infection.
Kit Components:H6008-95G1: Microtiter Plate, 1x96wells Coated with HIV Antigen H6008-95G2: Sample Diluent, 1x6mlH6008-95G3: HIVAg (HRP), 1x12mlH6008-95G4: Negative Control Serum, 1x1mlH6008-95G5: Positive Control Serum, HIV-1, 1x1mlH6008-95G6: Wash Buffer, 20X, 1x50ml Dilute 1:20H6008-95G7: Substrate A, 1x6mlH6008-95G8: Substrate B, 1x6mlH6008-95G9: Stop Solution, 1x6mlH6008-95G10: Positive Control Serum, HIV-2, 1x1ml
Storage and Stability:Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
