Hepatocyte culture mdium was designed and tested for our rat hepatocyte. This medium includes two components: Hepatocyte basal medium (H2004-70A1) and Hepatocyte culture medium supplement (H2004-70A2). The hepatocyte culture medium is a serum-free medium supplemented with BSA and several recombinant growth factors. Each lot of this product is tested in cell culture to ensure its sterility. This product does not contain antibiotics.
Use of Hepatocyte Culture Medium for Culturing Rat Hapatocytes:1. Thaw the supplement and add the entire volume (5ml) to the basal medium (500ml). Antibiotics may also be added if contamination is a concern
2. Take the amount of hepatocyte culture medium needed and warm to 37°C in a water bath or incubator. Avoid frequent temperature change to the entire bottle of medium. The hepatocyte culture medium is stable for at least 1 month stored at 4°C.
Storage and Stability:The hepatocyte basal medium is stored at 4°C and is stable for at least 6 months. A change in color or appearance of precipitate may indicate deterioration. The hepatocyte culture medium supplement is stored at 4°C and is stable for at least 6 months.
