Intended Use:Hepatitis E Virus, IgG (HEV), ELISA Kit, BioAssay™ is an in vitro enzyme immunoassay for the detection of Anti-HEV antibodies IgG in human serum or plasma. It is for diagnosis of early infection and epidemic survey.
Principle of the Test:This kit uses indirect ELISA method to detect anti-HEV antibodies IgG in serum or plasma. The purified activated recombinant HEV antigen (ORF2, ORF3) is coated on the multi-wells plate. The HRP conjugated mouse anti-human IgG (r chain) monoclonal antibody serves as tracer. TMB is substrate for HRP. The enzyme reaction with substrate TMB produces a color change, and the intensity of the absorbance at 450nm indicates the presence or absence of Anti-HEV antibodies IgG in the sample. The test is specific, sensitive, reproducible and easy to operate. It is for blood screen of HEV infection.
Recommended Instruments:8x12 wells plate reader and washer
