ELISA Kit for IgM Antibody to Hepatitis B Core Antigen is an in vitro enzyme immunoassay for the detection of Anti-HBc-IgM in human serum or plasma.
Principle:The purified Anti-mu-chain is coated on the solid phase of multi-wells. Serum sample, HBcAg and Horseradish peroxidase labeled with Anti-HBc (conjugated) are added to coated wells. After incubation, if Anti-HBc-IgM is present in the sample, a complex of Anti-mu-chain-Anti-HBc-IgM-HBcAg-Anti-HBc labeled with HRP will form. Wash wells to remove other unbounded serum components, incubate with substrates (TMB) to form a colored product, and measure the absorbance at 450nm to indicate the presence or absence of Anti-HBc-IgM in the sample. The test is special, sensitive, reproducible and easy to operate.
Kit Components:1. Anti-mu-chain Coated Microwell Plate2. Enzyme Conjugant3. Positive Control Serum4. Negative Control Serum5. Wash Buffer (1:20 dilution prior to use)6. Substrate A7. Substrate B8. Stop Solution9. HBcAg
Storage And Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
