Description:Mouse EPOC growth medium was designed and tested for our Mouse endothelium progenitor Outgrowth cells (mEPOC). This medium includes two components: Mouse EPOC basal medium (E3010-72G1) and Mouse EPOC growth medium supplement (E3010-72G2). The Mouse EPOC growth medium supplement includes fetal bovine serum and recombinant growth factors, and some lot-to-lot variability is unavoidable.
Each lot of this product is tested in cell culture to ensure its sterility. This product does not contain antibiotics.
Mouse EPOC growth medium was designed and tested for mouse Endothelial Progenitor Outgrowth Cells (mEPOC). The Mouse EPOC growth medium supplement has some lot-to-lot variability is unavoidable.
Each lot of this product is tested in cell culture to ensure its sterility. This product does not contain antibiotics
Use of EPC Growth Medium for Culturing EPOC:1. Thaw the supplement and add the entire volume (25ml) to the basal medium (500ml). Antibiotics may also be added if contamination is a concern
2. Take the amount of EPOC growth medium needed and warm to 37°C in a water bath or incubator. Avoid frequent temperature change to the entire bottle of medium. The Mouse EPC growth medium is stable for at least 1 month stored at 2-8°C
3. Refer to manual for EPOC culture procedures using the Mouse EPOC growth medium. We recommend changing medium every 2 days for regular cell culture.
Storage and Stability:The EPOC basal medium is stored at 4°C. A change in color or appearance of precipitate may indicate deterioration. The Mouse EPOC growth medium supplement is stored at -20°C. If taking aliquots from the original bottle is necessary, dispense the contents and re-freeze in small aliquots to avoid repeated freezing-thawing cycles. Mouse EPOC basal medium: Stable for 6 months from the date of receipt under proper storage condition. Mouse EPOC growth medium supplement: Stable for at least 1 year from the date of receipt under proper storage condition.
