The protein is known as the "building blocks of life" and is one of the most important macromolecules in life science. Proteins are polypeptides made up of amino acids and play various key roles in all aspects of biology. Protein quantitation is a very common practice for life scientists. Simple, direct and automation-ready procedures for measuring protein concentration are very desirable. Protein assay kit is based on an improved Coomassie Blue G method. The dye forms a blue complex specifically with protein, and the intensity of color, measured at 595nm, is directly proportional to the protein concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits increased sensitivity towards peptides.
Applications:Direct Assays: Total protein concentration
Key Features:Sensitive and accurate: Use 10ul samples. Detection range 0.06-1.0mg/ml protein in 96-well plate assay
Simple and high-throughput: The “mix-and-read” procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day
Low interference: Glucose, Tris, vitamins and amino acids, DNA, RNA, salts, EDTA (<12mM), phenol (<50mM), urea (<0.6M), Triton (<0.1%) and SDS (<0.1%) do not interfere in the assay
Versatility: Assays can be executed in 96-well plate or cuvet.
Kit Components:B2674-95A: Reagent (5X), 1x20ml. Supplied as a liquid in 48% Phosphoric acid, 25% Methanol, <0.05% Brilliant Blue G, <0.05% Triton X-100B2674-95B: Protein Standard, 1x1ml. Supplied as a liquid, 1mg/ml BSA
Storage and Stability:Store B2674-5A at 4°C in the dark. Store B2674-95B at -20°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
