The ZIP6 gene (LIV-1) was isolated in an effort to identify the estrogen-regulated genes in a human breast cancer cell line, ZR- 75-1. The mRNA expression of ZIP6 was upregulated about 4-fold in the presence of 10-8 M estradiol in culture medium. ZIP6 protein also contains a ubiquitin binding site.
ZIP6 rat: 741aa; human: 749aa; mouse: 765aa - 86kD; human Chromosome: 18q12.2. Highly expressed in the brain and testis. In the brain strongly expressed in the CA1 and CA3 regions, Purkinje cells in cerebellum and dentate gyrus in hippocampus. In testis found in spermatids or mature sperms in the central areas of seminiferous tubules.
Source:Synthetic peptide corresponding to 20aa of human ZIP7 (Accession #Q92504), N-terminal.
Molecular Weight:~3kD
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested.
Recommended Dilution:ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserumOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Synthetic peptide
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
