Triiodothyronine, Free, Human, BioAssay™ ELISA Kit (fT3, Free T3) is based on competitive binding of human free thyroxine from serum samples and enzyme-labeled T3 to T3-specific antibodies immobilized on microtiter well plates. After a washing step, chromogenic substrate is added and color developed. The enzymatic reaction (blue color) is inversely proportional to the amount of T3 present in the sample. The reaction is terminated by adding stopping solution (converts blue to yellow). Absorbance is then measured on a microtiter well ELISA reader at 450nm. and the concentration of free T3 in samples and control is read off the standard curve.
Sensitivity:0.3pg/ml.
Range:2-40pg/ml
Kit Components:T8425-06A1: Microstrip Plate, 1x96wellsT8425-06A2: Assay Buffer, 1x15mlT8425-06A3: Sample Diluent/Standard 0pg/ml 1x10mlT8425-06A4: Standard 2pg/ml 1x500ulT8425-06A5: Standard 4pg/ml 1x500ulT8425-06A6: Standard 8pg/ml 1x500ulT8425-06A7: Standard 16pg/ml 1x500ulT8425-06A8: Standard 40pg/ml 1x500ulT8425-06A9: Low Control (3.5-5.5pg/ml), 1x500ulT8425-06A10: High Control (6.3-10.5pg/m). 1x500ulT8425-06A11: Ab (HRP), 50X, 1x300ulT8425-06A12: Wash Buffer, 10X, 1x50mlT8425-06A13: TMB Solution, 1x16mlT8425-06A14: Stop Solution (1M sulfuric acid), 1x6ml
Storage and Stability:Store all components except standards and control at 4°C. Aliquot Standards and Controls storing at -20°C. Stable for a 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
