Steroids are a large group of complex tetracyclic lipids that consist of a 17-carbon-ring system. Examples are bile acids, sterols, various hormones and saponins. These hormones are powerful signal molecules that regulate a host of organismal functions.
Sterol regulatory element binding proteins (SREBPs) are membrane-bound transcription factors that control the metabolism of cholesterol and fatty acids in animal cells. Two SREBPs, designated SREBP-1 and SREBP-2, have been isolated and cloned from several mammalian species. Human SREBP-1 and -2 are ~ 50% identical in aa sequence. They share the tripartite structure, and they both have the capacity to activate the same genes. Although the two proteins can form heterodimers, this does not appear necessary for their function.
SREBP1: Rat- 1133aa; human-1147aa; mouse-1134aa;, ~121.6kD; Chromosome 11B2. Isoform SREBP-1C predominates in liver, adrenal gland, brain and adipose tissue, whereas isoform SREBP-1A predominates in spleen. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, thymus, testis, muscle, jejunum, and ileum.
Source:Synthetic peptide corresponding to 17aa of mouse SREBP1, C-terminal.
Molecular Weight:~3kD
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested.
Recommended Dilution:ELISA: coat plates at 1ug/ml and detected with antibodiesOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Synthetic peptide
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
