85-1561-27　RNP, Recombinant, Bovine, Western Blot Positive Control 100ul　R2995-05A

特徴

• nRNP Complex antigen is a non-histone nuclear protein composed of several polypeptides of differing molecular weights. It has been shown that RNP and Sm are distinct antigenic sites on the same nuclear molecular matrix consisting of RNA and eight polypeptides, including 70kD A(33kD), C(22kD) and the Sm peptides B (26kD), B'(27kD), D(13kD), E/F (11kD doublet), and G (9kD). Autoantibodies against the nRNP antigen precipitate the U1a and U1b small nuclear RNAs. The nRNP antigen is involved in normal post transcriptional, premessenger RNA processing to excise introns. It has been demonstrated that the nRNP antigen is both RNase and tryptic digestion sensitive. Autoantibodies to the nRNP antigen, in high titers, have been strongly associated with patients who have Mixed Connective Tissue Disease (MCTD). Other studies have shown that antibodies against the 70kD polypeptide are found more frequently in sera of MCTD than in anti-nRNP positive SLE patients. It has been suggested that patients showing anti-nRNP antibodies tend to have some of the clinical features of MCTD regardless of their overall disease diagnosis. Anti-nRNP antibodies only bind the U1 RNP while the anti-Sm binds with the U1 RNP, U2RNP, U4/6RNP, AND U5RNP. Anti RNP antibodies are often accompanied by anti-Sm antibodies. Double immunodiffusion, passive hemagglutination, Western and ELISA methods are used for detecting anti-nRNP.
• Source:Mixture of several proteins and associated RNAs (including 70kD, A(33kD), C(22kD) and the Sm peptides B (26kD), B'(27kD), D(13kD), E/F (11kD doublet), and G (9kD), purified from rabbit/calf thymus.
• Applications:Suitable for use in Western Blot. It is not suitable for ELISA or other applications where native protein is required. Other applications not tested.
• Recommended Dilution:Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.