85-1560-90　Maltose Binding Protein, Western Blot Positive Control 100ul　M2155-04C

特徴

• Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or c- terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enables a selective identification and purification of the protein of interest. The addition of a maltose binding protein (MBP) tag creates a stable fusion product that does not appear to interfere with the activity of the protein or with the cellular localization of the MBP- tagged product. The expression of polypeptides in-frame with maltose binding protein (MBP) allows for their easy, single- step purification from bacterial extracts under mild conditions using amylose resin. This system utilize a specific protease digestion site to facilitate correct cleavage of the fusion protein. Thus, the MBP system incorporates a factor Xa cleavage site at the carboxy terminus of the MBP sequence and cleavage by factor Xa separates MBP from its fusion protein. Many recombinant proteins have been engineered with MBP tags to facilitate the detection, isolation and purification of these proteins. Ant-MBP may be used in various immunoassay to identify the expression of a MBP fusion protein.
• Source:MBP, expressed in E.coli (containing a 23aa polylinker of pMAL- C2).
• Molecular Weight:~42kD
• Applications:Suitable for use in Western Blot. Not suitable for use in ELISA. Other applications not tested.
• Recommended Dilution:Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.