Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity. Recently, Ob genes (mouse and human) have been cloned. Obese gene encodes an adipocyte-tissue derived secreted Ob protein/Leptin (167aa, ~16kD) that controls body weight homeostasis. Exogenous administration of recombinant Ob protein can reduce food intake and body weight. However, Ob protein had no effect in db/db mice suggesting a defect in leptin signaling mechanism. Leptin exert its effects by interacting with leptin receptors (obese receptors). The Ob-R has been shown to be a product of db gene that has long been thought to encode the receptor for a weight-controlling hormone. The Ob-R has at least 6 alternatively spliced forms with modifications at the amino and C-terminus.
Human Leptin ELISA kit is based on binding of Human Leptin from samples to two antibodies, one immobilized on the microtiter well plates, and other bound to the enhancing protein Biotin, which then binds to strepavidin horseradish peroxidase conjugate. After a washing step, chromogenic substrate is added and colors developed. The enzymatic reaction (color) is directly proportional to the amount of Leptin present in the sample. Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450nm. and the concentration of Leptin in samples and control is read off the standard curve.
Storage and Stability:Store all components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
