A healthy non-immunized population of humans was used to collect serum. IgG was prepared by ammonium sulfate, and column chromatography (Sephadex, and DEAE-ion exchange columns). The resulting preparation was judged to be >95% pure by IEP and SDS-Page.
Source:Human normal serum.
Applications:Suitable for use in ELISA (coating), or as a non-immune control for ELISA, Dot Blot, Western Blot, and Immunohistochemistry. Other applications not tested
Recommended Dilution:ELISA: 10ug/mlDot Blot: 10ug/mlWestern Blot: 10ug/mlImmunohistochemistry: 10ug/mlOptimal dilutions to be determined by the researcher
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：1mg
Source Antigen：Human serum
Grade：Highly Purified
Purity：Highly Purified (~95%)
Form：Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 1ml sterile PBS.
