Human plasma was used for the purification of IgE. IgG was prepared by ammonium sulfate precipitation, column chromatography (Sephadex and DEAE-ion exchange columns) and affinity chromatography. The resulting preparation was judged to be >96% pure by IEP and SDS-Page. Purified IgE produced a single arc against anti-human serum and anti-human IgE. It does not react with anti-human IgA, IgD, IgG and IgM in IEP assay.
Applications:Suitable for use in ELISA (coating) or as a control for other immunological assay, Dot Blot, Western Blot. Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Extinction Coefficient:1.33 (0.1% at 280nm; 1cm path length)
Donor Consent:The raw material used in the purification of this product has been obtained from an FDA licensed collection center. Further the donor or the donor’s next of kin has given by signature full informed consent for the material to be used in commercial scientific research, including the sharing, transferring, or selling of samples to third parties involved in this research. The raw material was obtained in an ethical manner in full compliance with national legislation, and the donor identity is held as confidential information by the licensed collection center.
Testing:Shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
仕様
Size：100ug
Source Antigen：Human plasma
Grade：Highly Purified
Purity：≥95% (SDS-PAGE)
Form：Supplied as a liquid in 100mM Tris, pH 7.5, 200mM sodium chloride, 0.05% sodium azide
