Intended Use:The Human IgA ELISA Kit is an in vitro immunoassay for research use in the quantification of human IgA circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of human cells.
Test Principle:The Human IgA ELISA kit is based on the binding of human IgA in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgA present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgA in samples and control is calculated from a curve of standards containing known concentrations of IgA.
Kit Components:I1890-63A: Coated Microwell Plate: 12x8-well stripsI1890-63B: Human IgA Positive Control (245-455ng/ml), 1x 650ulI1890-63C: Human IgA Standard 50ng/ml, 1x 650ulI1890-63D: Human IgA Standard 100ng/ml, 1x 650ulI1890-63E: Human IgA Standard 250ng/ml, 1x 650ulI1890-63F: Human IgA Standard 500ng/ml, 1x 650ulI1890-63G: Human IgA Standard 1000ng/ml, 1x 650ulI1890-63H: IgA (HRP) Conjugate, 100X, 1x 150ulI1890-63J: Sample Diluent, 20X, 1x10mlI1890-63K: Wash Solution, 100X, 1x10mlI1890-63L: TMB Substrate: 1x12mlI1890-63M: Stop Solution: 1x12ml
Storage and Stability:Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
