Hexokinase catalyzes the first step of several metabolic pathways by converting D-hexose to D-hexose-6-P. Hexokinase is an allosteric enzyme inhibited by its products glucose-6-phosphate. At least 4 related hexokinase isoforms (HXKI-III; HXK-IV also known as Glucokinase) have been cloned and characterized. Hexokinases (~100kD for HXK1-III; HXKIV lacks the N-terminal domains and is ~50kD) are outer mitochondrial membrane proteins. The N-terminus, containing the mitochondrial target sequence, and the C-terminal has high sequence homology among various isoforms. The catalytic activity is associated with the C-terminus and other regulatory functions are controlled by the N-terminus. HXK3 (chromosome 5) has a more limited distribution that includes white cells and shows a genetically determined polymorphism. It is also expressed in lung and liver. It is 50% identical to HXK1.
Source:Synthetic 17aa peptide sequence from mouse HXK3, N-terminal.
Molecular Weight:~3kD
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested.
Recommended Dilution:ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserumOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Synthetic peptide
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
