85-1560-47　GST-mu, Recombinant, Human (Glutathione S-Transferase mu, GSTmu) 25ug　G8135-20K

特徴

• GST's are responsible for the metabolism of a broad range of xenobiotics and carcinogens. The enzyme catalyzes the reaction of glutathione with a wide variety of organic compounds to form thioethers, a reaction that is sometimes a first step in a detoxification process leading to mercapturic acid formation. Based on aa sequence similarities and antibody cross-reactivities, the mammalian cytosolic GSTs are divided into the following classes: alpha, mu, kappa, theta, pi, zeta, omega and sigma. GSTs exists as homodimers of ~25kD. GST A1-1 is mainly found in kidney, intestine, lung, and liver. GST M1-1 is restricted to liver and few other tissues. GST P1-1 has a widespread distribution in most tumor cells and tissues but it is absent in liver. The GST M1-1 is the type b allelic variant and corresponds to human GST Y.
• The GST M1 along with GST M2-GST M5 are mapped to Chrm 1, There is a close physical proximity between GSTM1 and GSTM2 loci, which shares 99% nucleotide sequence identity over 460 nucleotides of 3-prime untranslated mRNA. The 181aa long GST M1 is mainly expressed in liver, brain, testis, kidney and lung and show enzyme activity towards CDNB; AFB1 – epoxide; trans-4-phenyl-3buten-2-one.
• Source:Human GST-mu/GST M1-1, purified, expressed in E. coli.
• Molecular Weight:Under native conditions, GST-mu is homodimeric protein of ~50kD and under reducing conditions ~25kD.
• Specific Activity:~50units/mg
• Unit Definition:One unit will catalyze the conjugation of 1 micromole of 1-chloro-2-4-dinitrobenzene and reduced glutathione per min at pH 6.5 at 25°C.
• Applications:Suitable for use in biological activity determination, ELISA, and for standards or for positive control for Western Blot. Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.