85-1560-45　Glutathione Transferase-Alpha, Recombinant, Human, Western Blot Positive Control (GST-alpha, GST A1-1, Glutathione S-transferase A1, GST HA Subunit 1, GST Class-alpha Member 1, GST-epsilon, GSTA1-1, GTH1) 100ul　G8135-10B

特徴

• GST's are responsible for the metabolism of a broad range of xenobiotics and carcinogens. The enzyme catalyzes the reaction of glutathione with a wide variety of organic compounds to form thioethers, a reaction that is sometimes a first step in a detoxification process leading to mercapturic acid formation. Based on aa sequence similarities and antibody cross-reactivities, the mammalian cytosolic GSTs are divided into the following classes: alpha, mu, kappa, theta, pi, zeta, omega and sigma. GSTs exists as homodimers of ~25kD. GST A1-1 is mainly found in kidney, intestine, lung, and liver. GST M1-1 is restricted to liver and few other tissues. GST P1-1 has a widespread distribution in most tumor cells and tissues but it is absent in liver. The GST M1-1 is the type b allelic variant and corresponds to human GST Y.
• GSTA1: The alpha gene cluster consists of 5 genes GSTA1-GSTA5; the GSTA1 contains 222aa (chr 6p12), widely expressed in human tissues of liver, kidney, testis, adrenal and pancrease. GST alpha 1- 1 concentration as a marker for hepatocellular impairment, GSTA1 concentration in blood is a more specific marker than AST or ALT, and has a shorter half-life than ALT The GSTA1 shows enzyme activity towards CDNR; NBD-CI; delta 5 AD, PGE2 AND PGF2a synthase.
• Source:Human GST-alpha/GST A1-1 fusion protein with N-terminal His-tag (25kD), expressed in E. coli.
• Molecular Weight:Under native conditions, GST-alpha is homodimeric protein of ~50kD and under reducing conditions ~25kD.
• Applications:Suitable for use in Western Blot. Other applications not tested.
• Recommended Dilution:Western Blot: 10ul/lane. Heat once prior to loading on gels. The loading volume may be adjusted if necessary
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.