A sodium-dependent transport system is responsible for transfer and distribution of vitamins to different parts of the body, the transfer includes vitamins like pantothenate, biotin, and ascorbic acid etc, These transporters belong to Solute Carrier family (SLC). Since vitamins are required for essential metabolic processes in all mammalian cells, such cells have developed intrinsic mechanisms to active accumulation of essential vitamins. Thus transporters help these cells to fulfill their requirement, they include Sodium-dependent Multi-Vitamin Transporter (SMVT), Sodium-dependent Vitamin-C Transporter (SVCT) 1 & 2, Creatine Transporter (CRT1/ CT1). The other vitamin transporters from SLC family include Thiamine Transporter Protein 1 (THTR1), Folate Transporter or Reduced Folate Carrier 1 (RFC1), Thyroid Iodide Transporter (TIT) and Taurine Transporter (TAU) etc.
Folate Transporter/ FOLT/ RFC, a 512aa each protein in mouse and rat, 591aa in human (chrm 21q22.3, gene SLC19A1) transports folate compounds into mammalian cells via receptor mediated or carrier mediated mechanisms, as such maintains the intracellular concentration of folate. It shows 65% identity to mouse and hamster folate transporters.
Source:Synthetic peptide corresponding to 20aa of rat RFC, N-terminal, cytoplasmic domain.
Molecular Weight:~3kD
Applications:Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested.
Recommended Dilution:ELISA: coat plates at 1ug/ml and detected with antibodiesOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ug
Source Antigen：Synthetic peptide
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
