Fatty acids are important for general cellular metabolism. A number of proteins have been implicated in the transport and storage of fatty acid. FABPs (fatty acid binding proteins) are a group of cytoplasmic, small mol wt (14-15kD) and proteins that has wide spread tissue distribution. FABPs are quite abundant 3-5% of total cellular protein). At least 7 FABPs, FABP1-7, have been cloned and characterized from various tissues . FABPs can bind long-chain fatty acid, fatty - acid acyl - CoA and acyl-L-carnitine. Several different isoforms of FABP have been identified and generally referred to by tissue type (liver, heart, intestine, adipocyte, kidney, brain, etc; protein designated as H-FABP indicates that it is heart type). However , expression of these isoform is not exclusive and more than one isoform can be found in a given cell or tissue. Three main types of FABPs that were initially discovered in the heart (FABP- H), liver (FABP- L) , and intestine (FABP- I) are not exclusive these tissues and show considerable differences at the aa level (~30% identity). Other FABPs recently detected in adipocyte, kidney, and brain show a high degree of sequence homology among each other and with ot her FABPs. FABPs are also known as mammary derived growth inhibitor MDGI), adipocyte lipid binding protein (ALBP), Myelin protein P2 homolog, P2 adipocyte protein, 422 protein (P15). Human FABP-H or adipocyteis 132aa protein (chromosome 2p11) Rat and mouse adipocyte- FABPs are 133aa single polypeptide chains.
Cardiac fatty acid-binding protein (FABP) is a small cytosolic protein that is abundant in the heart and skeletal muscle. Following cardiac muscle injury it appears in the blood as early as 1.5h, peaks around 6h and returns to baseline values in 24h. Therefore, cardiac FABP serves as a useful biomarker for detection of heart disease.
Source:Porcine FABP protein from cardiac tissues.
Molecular Weight:~15kD
Applications:Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:50,000Western Blot: 1000-5000Immunohistochemistry: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ul
Source Antigen：Porcine heart
Grade：Purified
Purity：Highly Purified (~95%)
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
