Protein control for C7904-83 (affinity purified) or C7904-80H (serum).
Prostaglandins are important regulators of immune responses, fever, and pain. Two isoforms of Prostaglandin H synthase are well characterized, namely COX1 (also called PGHS-1; PHS-1; Prostaglandin-endoperoxide synthase-1) and COX2 (also called PGHS-2; Prostaglandin -endoproxide synthase-2 and PHSII). Both forms of COX proteins are membrane associated heme proteins containing Cyclooxygenase and peroxidase activities. These enzymes are targets of NSAID (Non steroidal anti-inflammatory drugs) such as aspirin. Cox-1 (human 599aa; rat 602aa) is homodimer of 70kD subunits. COX1 is constitutively expressed although significant enhancement of COX1 expression can be induced in some cell types. High expression is observed in gastrointestinal tissues. COX2 is a 72kD protein having 60% homology to COX1. It is induced by cytokines and mitogens and is likely to play a role in inflammatory diseases such as rheumatoid arthritis.
Source:Synthetic peptide corresponding to 17aa of human Cox-2, C-terminal.
Applications:Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ul
Source Antigen：Synthetic peptide
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
