Sulfated glycoprotein-2 (SGP-2) is the major secreted product of Sertoli cells and is thought to play a critical role in spermatogenesis. The protein was shown to be a normal constituent of human blood. It consists of two 40kD chains, alpha and beta, covalently joined by disulfide bonds. They established that SP-40,40 is a member of the human complement system by directly demonstrating its presence within the S-protein-containing soluble variant of the C5b-9 complex, SC5b-9. SP-40,40 is also called complement lysis inhibitor. It acts as a control mechanism of the complement cascade; specifically, it prevents the binding of a C5b-C7 complex to the membrane of the target cell and in this way inhibits complement-mediated cytolysis.
Apolipoprotein J is the human analog of the rat protein present in high concentrations in the testis, sulfated glycoprotein-2. It is a 70kD protein associated with high-density lipoproteins (HDL) in human plasma. There is a single copy of the APOJ gene in the human and mouse genomes. The protein is synthesized as a 427aa polypeptide that is posttranslationally cleaved at an internal bond between arg205 and ser206. Two subunits, designated alpha (34 to 36kD), corresponding to residues 1-205, and beta (36 to 39kD), corresponding to residues 206-427, are associated through disulfide bonds. APOJ mRNA (1.9kb) in all but one tissue examined. Its concentration was relatively high in brain, ovary, testis, and liver, lower in heart, spleen, lung, and breast, and absent in T lymphocytes. Apolipoprotein J is distinct from other known apolipoproteins in molecular weight, subunit structure, and isoelectric point.
ApoJ is induced in myocarditis and numerous other inflammatory injuries. Deficient and wildtype mice exhibited similar initial onset of myocarditis. Furthermore, autoantibodies against the primary antigen cardiac myosin were induced to the same extent. After resolution of inflammation, apoJ-deficient, but not wildtype, mice exhibited cardiac function impairment and severe myocardial scarring. These results suggested that apoJ normally limits progression of autoimmune myocarditis and protects the heart from postinflammatory tissue destruction.
Source:Recombinant human ApoJ expressed with N- terminal fusion of T7-Tag (16aa) and C-terminal fusion of His-Tag (9aa), expressed in E.coli.
Molecular Weight:~26kD
Applications:Suitable for use in Western Blot. Not suitable for use in ELISA. Other applications not tested.
Recommended Dilution:Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ul
Source Antigen：Recombinant, E.coli
Grade：Purified
Purity：~95% (SDS-PAGE)
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
