85-1559-99　Citrate Synthase, Mitochondrial, Porcine, Western Blot Positive Control 100ul　C5700-04E

特徴

• The enzyme citrate synthase (E.C. 2.3.3.1 [previously 4.1.3.7]) is a pace-maker enzyme, as it controls the first committed step of the Krebs cycle, also called the citric acid cycle. Citrate synthase is localized within cells in the mitochondrial matrix, but is encoded by nuclear DNA rather than mitochondrial. It is synthesized using cytoplasmic ribosomes, then transported into the mitochondrial matrix. Citrate synthase is commonly used as a quantitative enzyme marker for the presence of intact mitochondria. Citrate synthase catalyzes the condensation reaction of the two-carbon acetate residue from acetyl coenzyme A and a molecule of four-carbon oxaloacetate to form the sixcarbon citrate. Oxaloacetate will be regenerated after the completion of one round of the Krebs Cycle.
• Human CS (chromosome 12p11-qter, 466aa precursor, mature chain 28-466aa) encodes a 466aa protein with 95% homology to its pig homolog. CS enzyme, a homodimer, is located at the Mitochondrion matrix. Citrate synthase is found in nearly all cells capable of oxidative metabolism.
• Source:Citrate synthase protein from pig heart.
• Applications:Suitable for use in Western Blot. Not suitable for use in ELISA. Other applications not tested.
• Recommended Dilution:Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.