85-1559-85　Apolipoprotein H, Western Blot Positive Control (Beta-2-glycoprotein 1, APC Inhibitor, Activated Protein C-binding Protein, Anticardiolipin Cofactor, Apolipoprotein H, Apo-H, Beta-2-glycoprotein I, B2GPI, Beta(2)GPI, B2G1) 100ul　A2299-77Y

特徴

• Beta-2 Glycoprotein (also known as BETA-2-Glycoprotein I; B2G1 BG; Apolipoprotein H,(Apo-H); (B2GPI) (Beta(2)GPI) (Activated protein C-binding protein) (APC inhibitor) (Anticardiolipin cofactor ) is a 50kD glycoprotein (human 345aa, chromosome 17q23) that is synthesized in the liver and secreted into blood (~200ug/ml). B2GP is a carbohydrate-rich plasma protein that is a constituent of several lipoprotein particles. It contains sialylated and galactosylated bi- and triantennary oligosaccharides.
• B2GP Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. It may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. B2GP is a member of the complement control proteins (CCPs or Sushi domains). It consists of 5 CCP repeats in which the first 4 domains are regular repeats consisting of 60aa. The fifth domain is different from the other 4 domains as it consists of 82aa, 4 conserved hydrophobic aa313-316, 3 disulfide bonds instead of 2, and a cluster of positively charged aa282-287, which is responsible for binding of B2GPI to phospholipids.
• Source:Purified human B2M protein from plasma.
• Applications:Suitable for use in Western Blot. Not suitable for use in ELISA or Immunoprecipitation. Other applications not tested.
• Recommended Dilution:Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.