Human Serum Amyloid A protein-1 (SAA1) is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12kD, 104aa, nonglycosylated polypeptide that displaces apoA1 in the HDL3 complex. The SAA1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA1 gene product differs from the SAA2 gene product by only 7aa. Circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three aas being cleaved from either the N- or C-terminus. Mature human SAA1 is 72%, 82% and 72% aa identical to mature mouse, rabbit and hamster SAA1, respectively.
Source:Purified human SAA protein.
Molecular Weight:~2kD
Applications:Suitable for use in Western Blot. Not suitable for use in ELISA or other applications where native protein is required. Other applications not tested.
Recommended Dilution:Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
仕様
Size：100ul
Source Antigen：Human
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
